Rhoda Makoff received her PhD in Biochemistry in 1961 when she was 23 years old. Our conversation explores how Rhoda developed an interest in science and how she built a successful career in academic research, teaching, and business despite the continual roadblocks that she encountered as a woman who envisioned a major career. Rhoda reflects on the principles that have guided her resilience and persistence in achieving her goals, including the importance of keeping your eyes on the prize and developing an egoless style of leadership.